Coding

Part:BBa_K1475002:Design

Designed by: Daniel Weltz Pedersen   Group: iGEM14_SDU-Denmark   (2014-08-22)

FAT-2 delta 12 desaturase from C. elegans


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 117
    Illegal BamHI site found at 253
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 169
    Illegal SapI site found at 582


Design Notes

Synthesized from the coding sequence of isoform a, sequence avaliable on wormbase.org. [1] Prefix and suffix were added using the following primers.
Forward:
AAGAATTCGCGGCCGCTTCTAGATGACAATCGCTACAAAAGTGAAC
Rewerse:
AAGAATTCGCGGCCGCTTCTAGATGACAATCGCTACAAAAGTGAAC


Source

Organizm: Caenorhabditis elegans
The gene was synthesized based on the cDNA sequence from wormbase.org and was generously provided by Julius Fredens from University of Southern Denmark. [2]

References

1. Xue-Rong Zhou, et al.: Caenorhabditis elegans Δ12-Desaturase FAT-2 Is a Bifunctional Desaturase Able to Desaturate a Diverse Range of Fatty Acid Substrates at the Δ12 and Δ15 Positions. J Biol Chem. Dec 23, 2011; 286(51): p. 43644–43650, http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3243535/
2. Wormbase, 10 Jun 2010: fat-2. http://www.wormbase.org/species/c_elegans/gene/WBGene00001394?query=fat-2#0-9e-3.
3. Uniprot, fat-2_CAEEL accession number: G5EGA5. http://www.uniprot.org/uniprot/G5EGA5